Extended Data Fig. 3: βarr conformational changes by V2R phsphopeptide using Fab30.
From: Membrane-domain compartmentalization of active GPCRs by β-arrestins through PtdIns(4,5)P2 binding
(a) Representative western blot imaging for the co-immunoprecipitation analysis. The indicated βarr protein constructs were subjected to the Fab30 pull-down assay in presence of the V2Rpp phosphopeptide. (b) Quantification of the data in (a). Bars and error bars represent mean and SEM, respectively, of three independent experiments. For the statistical analyses, data were analyzed by one-way ANOVA followed by the Dunnett’s test for multiple comparison analysis. ***P < 0.001 vs. WT.
