Extended Data Fig. 4: LC/Fourier transform ion cyclotron resonance (FT-ICR) MS detection of sulfated T3, the known SULT1B1 substrate.
From: Mass spectrometry and enzyme assays refute histone tyrosine sulfation
Activity of rhSULT1B1 was optimized using its known substrate, T3, and LC/FT-ICR MS analysis achieved via positive polarity mode. Extracted ion chromatograms of sulfated T3 (a) showed an abundance of 8e8, while non-sulfated T3 (b) was at 2.5e9 abundance after incubation with rhSULT1B1 and PAPS at 37 °C overnight. Please note that sulfation reduces ionization efficiency in positive ion mode and, thus, the relative abundance in the chromatogram is not representative of actual concentrations.
