Fig. 2: A>I snRNAs perturb fewer genes than cadRNAs.
From: Enhancing RNA base editing on mammalian transcripts with small nuclear RNAs
a, Scatter plots of differential gene expression analysis against empty control (pUC19) of cadRNA backbone versus U7smOPT snRNA backbone for RAB7A-targeting and DMD-targeting guides. Cutoffs for significance: |log2(fold change)| > 0.5 and adjusted P value < 0.05. Upregulated genes are colored in red and downregulated genes are colored in blue (n = 2 biological replicates per condition). b, Left: number of LSV events from differential splicing analysis against empty control (pUC19) of cadRNA backbone versus U7smOPT snRNA backbone for RAB7A-targeting and DMD-targeting guides, with P < 0.05 and different thresholds of dPSI for events. Right: four-way Venn diagrams of the number of significantly upregulated and downregulated splicing factors represented in the significantly perturbed genes from a (n = 2 biological replicates per condition).
