Fig. 3: A>I snRNAs localize more persistently to the nucleus.
From: Enhancing RNA base editing on mammalian transcripts with small nuclear RNAs
a, Left: schematic of subcellular localization qPCR to determine nuclear-to-cytosolic enrichment of A>I snRNA and cadRNA A>I base editors. Right: results from subcellular localization qPCR of the A>I base editors with guides targeting three different genes, using the lncRNA NEAT1 as a positive control for the assay. Nuclear-to-cytosolic enrichment significance versus cadRNA: *P < 0.05 and ***P < 0.001 (one-way ANOVA) (RAB7A, 0.0121; GAPDH, 5 × 10−4; TARDBP, 0.0216). Error bars reflect the s.e.m. (n = 3 biological replicates per condition). b, RCA FISH schematic, representative images and plots of rolonies per cell, mean rolony distance to nucleus and nuclear-to-cellular rolony ratio for GAPDH-targeting A>I snRNA and cadRNA. RCA FISH was performed in human U-2 OS cells with ×10 magnification images. Images depicting fluorescence signal are small representations of the total number of nuclei (thousands per biological replicate) and are not suitable for visual interpretation. Pixel resolution: 752 nm × 752 nm. Significance of difference: *P < 0.05 and **P < 0.01 (one-way ANOVA) (rolonies per cell, 0.0191; mean rolony distance to nucleus, 0.0016; nuclear-to-cellular rolony ratio, 0.0051). Error bars reflect the s.e.m. (n = 3 biological replicates per condition).
