Extended Data Fig. 4: LIC1 is associated with the progression of NSCLC.
From: A small molecule targets LIC1 to suppress lung tumor growth by inducing autophagy
a, Pull-down experiments of DAA-PT (5 μM) on different LIC1 mutants in 293 T cells. 3 A: Tyr93, Lys94 and Glu100 mutate into Ala; 5 A: Gly96, Arg97, Leu99, Glu100, Tyr101 and Trp120 mutate into Ala. b, Cell counting assay after LIC1 overexpression in H1975 and HCC827 cells. c, Immunoblotting analysis of the indicated proteins in H1975 and HCC827 cells overexpressing LIC1 or corresponding vector. d,e, H1975 and HCC827 cells stably overexpressing LIC1 or corresponding vector were subcutaneously transplanted in nude mice (n = 6 biologically independent mice per group). Tumor growth curves, subcutaneous tumors at the experimental endpoint and tumor weight are shown as indicated. Tumor volume is presented as mean values ± s.e.m., tumor weight is shown as mean ± s.d. Statistical significance was determined by two-tailed unpaired Student’s t-test, and representative tumor images are shown. f, Immunohistochemistry images and quantification of Ki67 expression in tumors from d and e. Scale bars, 100 μm. g, Immunoblot of V5 in H1975 cells infected by PLX304-LIC12A overexpressing or control PXL304 lentiviruses after 72 h. Data in b and f are shown as mean ± s.d. Statistical significance was determined by two-tailed unpaired Student’s t-test. For a–c, f and g, n = 3 independent experiments.
