Extended Data Fig. 2: DAA induces autophagy-mediated cell death.
From: A small molecule targets LIC1 to suppress lung tumor growth by inducing autophagy
a, Representative western blot for cycle-related proteins in H1975 and HCC827 cells treated with indicated concentrations of DAA for 24 h. b, H1975 cells were exposed to indicated concentrations of DAA with or without 3-MA (60 μM) for 48 h. Then the cell viability was assayed. c, H1975 cells and HCC827 cells were exposed to DAA (1.25 μM for H1975 cells or 2.5 μM for HCC827 cells) alone or in combination with Autophinib (50 nM) and SBI-0206965 (0.5 μM), respectively. After 48 h of treatment, the cell viability was assayed. Right panels show the corresponding immunoblots. The ratio of LC3-II: GAPDH was calculated based on the band density. d, Representative western blot for the indicated proteins in H1975 and HCC827 cells treated with DAA (2.5 μM) for indicated time points. e, H1975 cells were treated with DAA (2.5 μM), Baf (0.5 μM), or Rap (1 μM) for 4 h, followed by staining with LysoTracker Red (LTR, 50 ng/mL) or acridine orange (AO, 0.5 μg/mL) for 30 min. Fluorescence images of live cells were recorded without fixation. Scale bars, 10 μm. Data in b and c are shown as mean ± s.d. Statistical significance was determined by two-tailed unpaired Student’s t-test. For a–e, n = 3 independent experiments.
