Extended Data Fig. 3: DAA directly targets LIC1.
From: A small molecule targets LIC1 to suppress lung tumor growth by inducing autophagy
a, Cell viability curves measured by CCK-8 for H1975 cells treated with probes for 72 h. b, Western blot analysis on endogenous LIC1 and LIC2 protein after DAA-PT pull-down in HCC827 cells. c, Western blot analysis on exogenous LIC1 protein after DAA-PT pull-down in 293 T cells. 293 T cells were transfected with V5-tagged LIC1 for 6 h. After 24 h, the cells were incubated with indicated concentrations of DAA-PT or IN-PT in the presence or absence of DAA for 3 h. After photocross-linking, the probe-bound protein was precipitated by streptavidin beads and was immunoblotted with an antibody against V5. d, Representative western blot for LIC1 in indicated cells treated with indicated concentrations of DAA for 24 h or treated with DAA (2.5 μM) for indicated time points. e, Quantification for LIC1 protein levels in the cellular thermal shift assay. Statistical significance was determined by two-tailed unpaired Student’s t-test compared with vehicle group. f, SPR analysis of interactions between 20 and LIC1. Data in a and e are shown as mean ± s.d. For a–f, n = 3 independent experiments.
