Extended Data Fig. 7: Stability and degradation analysis of NEtrap1080@LNP in vivo.
From: Designing chemigenetic DNA nanotrap for norepinephrine dynamic imaging in organelles
a, Schematic diagram of NEtrap1080. b, Agarose gel electrophoresis analysis of the assembly of NEtrap1080. I-IX represent A-CHO, B-BAN, C-BAN, D-FD1080, A(CHO)B(BAN), A(CHO)B(BAN)C(BAN), A(CHO)B(BAN)C(BAN)D(FD-1080) and NEtrap1080. c, MALDI mass spectrum of NEtrap1080. d, (I) UV-vis absorption spectrum and (II) fluorescence emission spectrum of NEtrap1080. e, Schematic diagram of NEtrap1080@LNP. f, TEM image of NEtrap1080@LNP. Three independent experiments yielded similar results. g, Confocal image of NEtrap1080@LNP collected from fluorescence signal between 400-500 nm. h, FT-IR spectra of (I) NEtrap1080, (II) LNP, (III) Xen2174 and (IV) NEtrap1080@LNP. i, In vivo whole-body fluorescence imaging at different time points after NEtrap1080@LNP was injected into the brain. Three independent experiments yielded similar results. j, Quantitative analysis of in vivo fluorescence imaging intensity in (i). Data are presented as mean ± S.D. Error bars: S.D., n = 3 independent experiments. k, Fluorescence images of various organs at different time points after NEtrap1080@LNP was injected into the LC region of mice brain. Three independent experiments yielded similar results.
