Extended Data Fig. 2: Neuronal types analysis.
From: Designing chemigenetic DNA nanotrap for norepinephrine dynamic imaging in organelles
a, Fluorescence images of the neurons obtained from (I) DAPI signal (nuclear marker, blue color), (II) DBH signal (NE neuron marker, cyan color), (III) probe signal (red color) and (V) merged signals. b, Immunoblotting analysis of (I) DBH and (II) GAPDH in the used neurons obtained from LC region of mice. c, Co-localization imaging of neurons pre-transfected with (I) membrane-targeted, (II) endoplasmic reticulum-targeted, and (III) mitochondrial-targeted HaloTag proteins, respectively. The neurons were co-stained with NEtrapHTL and commercial organelle-targeted probes. Hoechst, CellLight™ PM-RFP, CellLight™ ER-RFP, and CellLight™ Mito-RFP were used to target nucleus, PM, ER, and Mito, respectively. Three independent experiments yielded similar results.
