Extended Data Fig. 1: Polyamines form condensates with heparin.
From: Mast cell extracellular granules are bioactive condensates assembled by heparin and polyamine
a, Fluorescent images of Cy3-labeled heparin in the presence of increasing concentrations of spermine in 50 mM HEPES, pH 7.4 buffer related to Fig. 1c. Scale bar, 10 μm. b, Turbidity of heparin-spermine condensate at varying pH. Condensates were assembled by 100 μM heparin and 10 mM spermine in Na2HPO4-citrate buffer at the indicated pH. N = 3 biological replicates. P value by ordinary one-way ANOVA. c, Condensation of varying heparin and chondroitin sulfate concentrations in the presence of spermine. Scale bar, 10 μm. Data are presented as Mean ± SD. One representative experiment from 3 independent experiments was shown.
