Supplementary Figure 2: Flow cytometry analysis of B and T cell development in Hoxb5-overepressing transgenic mice

(a) Schematic diagram of Hoxb5 knock in mouse model. The Hoxb5-EGFP expression cassette was inserted into ROSA26 (Hoxb5^LSL/+ mice). (b) Flow cytometry analysis of CD19⁺B220⁺IgM^hiIgD^lo immature B and CD19⁺B220⁺IgM^loIgD^hi mature B cells in BM, Spleen and PB of Hoxb5^LSL/+ CD19-Cre and littermate Hoxb5^LSL/+ control mice (8-week-old). Representative plots were shown. (c) Flow cytometry analysis of CD19⁺B220⁺CD93⁺IgM^- pro-pre-B cells in the bone marrow of Hoxb5^LSL/+ CD19-Cre and littermate Hoxb5^LSL/+ control mice (8-week-old). Representative plots were shown. (d-e) Flow cytometry analysis of DN cells in thymus (d) gated from Ter119^-Mac1^-CD19^- population, T cells in PB, Spleen, and LN (e) gated from Ter119^-Mac1^- population of Hoxb5^LSL/+Vav-Cre mice and littermate control (8-week-old mice). Representative plots of DN cells in thymus and T cells in PB, Spleen, LN and BM were shown. (f) Flow cytometry analysis of Lin^-CD44⁺c-kit^hiCD25^- early T cell lineage progenitors (ETP) in thymus and BM from the Hoxb5^LSL/+Vav-Cre mice and littermate control (8-week-old mice). Representative plots were shown. Data are representative of two independent experiments (b-f).