Supplementary Figure 3: Competitive bone marrow transplantation using Hoxb5-overepressing transgenic mice or Hoxb5-deficent transgenic mice as donors
From: Transcription factor Hoxb5 reprograms B cells into functional T lymphocytes
(a-c) Flow cytometry analysis of hematopoietic lineages in PB (a), BM (b), and spleen (c) of representative Hoxb5LSL/+Vav-Cre and Hoxb5LSL/+ mice (12-week-old). (d) Flow cytometry analysis of Lin-CD127+c-kitintSca-1int common lymphoid progenitors (CLP) in bone marrow of representative Hoxb5LSL/+Vav-Cre and Hoxb5LSL/+ mice. (e) Flow cytometry analysis of double negative (DN) cells in thymus gated from Ter119-Mac1-CD19-CD45.2+ population. Hoxb5LSL/+Vav-Cre and Hoxb5LSL/+ mice were analyzed. (f) Competitive transplantation analysis of Hoxb5LSL/+Vav-Cre group and Hoxb5LSL/+ (Ctr group). Half million total bone marrow cells from either Hoxb5LSL/+Vav-Cre or Hoxb5LSL/+ mice (CD45.2+) with equivalent number of competitor cells (CD45.1+) were retro-orbitally transplanted into lethally irradiated (9 Gy) individual CD45.1+ recipients. Donor contributions in PB of recipient mice were shown. Control group (n = 5 mice), and Hoxb5LSL/+Vav-Cre group (n = 11 mice). Donor chimerism for total cells, Mac1+ myeloid cells,,CD19+ B cells and CD3+ T cells were shown respectively. Each symbol represents an individual mouse, and small horizontal lines indicate the mean (± s.d.). (g-i) Flow cytometry analysis of hematopoietic lineages in PB (g), BM (h), and spleen (i) of representative Hoxb5-/- and wild type littermate mice (12-week-old). (j) Flow cytometry analysis of Lin-CD127+c-kitintSca-1int CLP in bone marrow of representative Hoxb5-/- and wild type littermate mice (12-week-old). (k) Flow cytometry analysis of DN cells in thymus gated from Ter119-Mac1-CD19-CD45.2+ population of representative Hoxb5-/- and wild type littermate mice (12-week-old). (l) For competitive transplantation, half million total bone marrow cells from either Hoxb5-/- or WT mice (CD45.2+) with equivalent number of competitor cells (CD45.1+) were retro-orbitally transplanted into lethally irradiated (9 Gy) individual CD45.1+ recipients. Donor contributions in peripheral blood of recipient mice were shown. Control group (n = 5 mice), and Hoxb5-/- group (n = 7 mice). Donor chimerism for total cells, Mac1+ myeloid cells, CD19+ B cells and CD3+ T cells were shown respectively. Each symbol represents an individual mouse, and small horizontal lines indicate the mean (± s.d.). Data are representative of three independent experiments (a-e, g-k).
