Supplementary Figure 4: PCR bands of B cell-specific BCR IgH V(D)J rearrangements and T cell-specific TCRβ V(D)J rearrangements in sorted single iT cells | Nature Immunology

Supplementary Figure 4: PCR bands of B cell-specific BCR IgH V(D)J rearrangements and T cell-specific TCRβ V(D)J rearrangements in sorted single iT cells

From: Transcription factor Hoxb5 reprograms B cells into functional T lymphocytes

Supplementary Figure 4

(a-c) PCR of B cell-specific VHJ558 and VHQ52 rearrangements and T cell-specific Vβ2-DJβ2, Vβ4-DJβ2, Vβ5.1-DJβ2 and Vβ8-Jβ2 rearrangements in sorted single iT cells of recipients transplanted with (a) retro-Hoxb5 pro-pre-B cells (Retro-iT), (b) single iT cells of CD19-Hoxb5 pro-pre-B recipients (KI-iT) and (c) single iT cells of Tet-Hoxb5 pro-pre-B recipients (Dox-iT) four weeks after transplantation. Wild-type bulk pro-pre-B cells and T cells were used as positive controls (PC). Water was used as DNA template negative control to exclude DNA contaminants (NC). 200 ng DNA of each amplified single lymphocyte genome was used as template for PCR of BCR IgH V(D)J and TCRβ V(D)J rearrangements.

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