Supplementary Figure 5: iT cells generated from Rag1^-/- mice exhibit immune functions

(a) Three million sorted GFP⁺ pro-pre-B cells from CD19-Hoxb5 transgenic mice were transplanted into individual Rag1^-/- recipient mice lacking mature T cells. Flow cytomerty analysis of PB were performed three weeks post-transplantation. Plots of three representative recipients from ten animals of three independent experiments were shown. (b) Images of allogeneic skin-grafted Rag1^-/- and Hoxb5-Rag1^-/- mice. Representative images of rejected allogeneic skin tissues from three mice (day 8) and successfully grafted skin tissue control (day 15) were taken. Three weeks before the skin transplantation, three million sorted GFP⁺ pro-pre-B cells from Hoxb5^LSL/+CD19-Cre mice were transplanted into individual Rag1^-/- mice. Donor skin tissues were from BALB/c mice.