Supplementary Figure 7: Flow cytometry analysis of LSK and CLP cells in recipient mice, and T development after thymic transplantation

(a) Flow cytometry analysis of phenotypic CD2^-CD3^-CD4^-CD8^-B220^-Mac1^-Gr1^-Ter119^- (Lin^-)c-kit⁺Sca-1⁺ cells (LSK) and Lin^-CD127⁺c-kit^intSca-1^int (CLP) cells in the bone marrow of 15-TF pro-pre-B cell recipients. (b) Flow cytometry analysis were performed on phenotypic CD2^-CD3^-CD4^-CD8^-B220^-Mac1^-Gr1^-Ter119^- (Lin^-)c-kit⁺Sca-1⁺ cells (LSK) and Lin^-CD127⁺c-kit^intSca-1^int (CLP) cells in the bone marrow of retro-Hoxb5 mice. (c) Flow cytometry analysis of phenotypic CD2^-CD3^-CD4^-CD8^-B220^-Mac1^-Gr1^-Ter119^- (Lin^-) c-kit⁺Sca-1⁺ cells (LSK) and Lin^-CD127⁺c-kit^intSca-1^int (CLP) cells in the bone marrow of CD19-Hoxb5 pro-pre-B cell recipient mice. (d) Flow cytometry analysis of phenotypic CD2^-CD3^-CD4^-CD8^-B220^-Mac1^-Gr1^-Ter119^- (Lin^-)c-kit⁺Sca-1⁺ cells (LSK) and Lin^-CD127⁺c-kit^intSca-1^int (CLP) cells in the bone marrow of recipients 4 weeks post-transplantation with BFP⁺ Tet-Hoxb5 pro-pre-B cells. The recipient mice were maintained on drinking water containing 1 mg/ml doxycycline one day prior to transplantation and for continuous two weeks. Data are representative of three independent experiments (a-d). (e-g) Flow cytometry analysis of DN cells in thymus (e) gated from Ter119^-Mac1^-CD19^- population, T cells in lymph node (f), spleen and bone marrow (g) gated from Ter119^-Mac1^-CD19^- population of recipients three weeks after thymic transplantation. A quarter of total wild type thymocytes (CD45.1⁺) were transplanted into sublethally irradiated individual recipients (CD45.2⁺) by intra-thymus injection. Data are representative of two independent experiments (e-g).