Supplementary Figure 8: Flow cytometry analysis of iT lymphocytes in Bio-Hoxb5 pro-pre-B recipient mice, and Runx2 and Smarca5 CHIP-Seq peaks

(a) Schematic diagram of Bio-tagged Hoxb5 vector cassettes. Bio-tagged Hoxb5 sequence was constructed into the pMY-IRES-GFP retro-vector. (b) Flow cytometry analysis of intra-cellular biotin in GFP control virus or Bio-Hoxb5 virus transduced pro-pre-B cells isolated from Rosa26^BirA/BirA transgenic mice. Cells were analysed 72 hours post-virus transplantation. (c-d) Flow cytometry analysis of T cells in PB, LN and Spleen (c) gated from Ter119^-Mac1^-GFP⁺ population and DN cells in thymus (d) gated from Ter119^-Mac1^-CD19^- GFP⁺ population of Bio-Hoxb5 pro-pre-B recipient mice six weeks post-transplantation. (e) ChIP-Seq profiles Hoxb5 binding tracks in pro-pre-B on Runx2 and Smarca5 gene locus. Data are representative of two independent experiments (b-e).