Supplementary Figure 1: Flow cytometry analysis of B cell development in recipients transplanted with 15-TF virus mixture transduced pro-pre-B cells

(a) Flow cytometry analysis of the purity of sorted Ter119^-Mac1^-CD3^-CD4^-CD8^-CD19⁺B220⁺CD93⁺IgM^- pro-pre-B cells. (b) Schematic diagram of 15-TF virus transduced pro-pre-B cells transplantation strategy. (c) Flow cytometry analysis of donor derived CD19⁺B220⁺IgM^hiIgD^lo immature B and CD19⁺B220⁺IgM^loIgD^hi mature B cells in bone marrow (BM), spleen and peripheral blood (PB) of 15-TF pro-pre-B cell recipient mice (CD45.1⁺ C57BL/6) four weeks post-transplantation. (d) Statistical analysis of immature and mature B cells in BM, Spleen, and PB. Each symbol represents an individual mouse, and small horizontal lines indicate the mean (± s.d.). *P < 0.05, **P < 0.01, ***P < 0.001 (two-sided-independent Student’s t-test), n = 3 biological replicates. (e) Flow cytometry analysis of donor derived CD19⁺B220⁺CD93⁺IgM^- pro-pre-B cells in the bone marrow of 15-TF mice. (f) Statistical analysis of donor derived pro-pre-B cells in BM of 15-TF pro-pre-B cell recipient mice (CD45.1⁺ C57BL/6). Each symbol represents an individual mouse, and small horizontal lines indicate the mean (± s.d.). ***P < 0.001 (two-sided-independent Student’s t-test, n = 3 biological replicates). Data are representative of two independent experiments (c, e).