Supplementary Figure 7: Human Treg and Tconv single-cell datasets: Quality-control plots and definition of the core human Treg transcripts.
From: Single-cell gene expression reveals a landscape of regulatory T cell phenotypes shaped by the TCR
a. Scatterplot matrix comparing the collapsed gene expression profiles of the Treg and Tconv scRNAseq replicates, with Pearson correlation and P value (t test). b. Scatterplot matrix comparing the variability in gene expression (Fano Factor = variance/mean) within each of the different Treg and Tconv scRNAseq replicates, with Pearson correlation and P value (t test). c. Treg vs Tconv changes in the gene expression in pooled scRNAseq (x-axis) compared with the changes in an independent population microarrray experiment (y-axis)53. Highlighted genes are genes are the canonical Treg signature genes. d. Same tSNE plots of human Treg and Tconv as in Fig. 7b, but split to show the contribution of each donor. e. Core human Treg signature. The same analysis as described in Fig. S2 was performed. This scatterplot compares the average fold change in gene expression (x axis) with the fraction of tests (y axis) that were significantly differently expressed between human Tregs and Tconvs (similar to Supplementary Fig. 3a). f. Nr4a1-GFP expression distribution in Tregs isolated from the blood and spleen of three mice.
