Supplementary Figure 2: IL-12 and in parts IL-1β, but not IFN-β are required for EC-induced TFH cell differentiation.
From: Recognition of microbial viability via TLR8 drives TFH cell differentiation and vaccine responses
(a) Proliferation of CD4+ T cells cultured as in Figure 4 in the presence of conditioned APC supernatants was measured by flow cytometry (CFSE dilution). (b, c) Same samples as in Figure 4a,b respectively. IL-17A production was measured by flow cytometry (b, n=28) and ELISA (c, n=10). (d-f) CD4+ T cells were cultured and stimulated as in Figure 3d (aIL-12; anti-IL-12 antibodies etc., rIL-12; recombinant IL-12 etc.). Bcl6 and IL-21 co-expression were measured by flow cytometry (d, n=4) and quantified (e, n=4), IL-21 production was quantified by ELISA (f, n=7). One-way ANOVA with post hoc correction for multiple comparison. Error bars are mean ± SEM. (*; p<0.05, **; p<0.01).
