Supplementary Figure 3: Endosomal receptors are required for the production of IL-12 in response to EC.
From: Recognition of microbial viability via TLR8 drives TFH cell differentiation and vaccine responses
(a) Human monocytes were left untreated (-) or treated with cytochalsin-D (CytD) to block actin polymerization and phagocytosis, or with bafilomycin A (Baf) to inhibit phagolysosomal acidification. Cells were subsequently stimulated with EC or HKEC. IL-6 and IL-12p40 release was measured by ELISA (n=3). (b) Expression of genes encoding for TLR1-9 was measured by qPCR in purified human monocytes, and expressed semi-quantitatively as Ct-ratio of house keeping gene beta-actin/Tlr (n=2). (c) Human monocytes were left untreated (ctrl), treated with pLA or pLA plus bacterial RNA (pLA+RNA) and upregulation of activation markers was measured by flow cytometry (n=5). Error bars are mean ± SEM.
