Supplementary Figure 4: Bacterial RNA induces BCL6/IL-21 expression, ST and HKST equally induce TBET and FOXP3 expression. | Nature Immunology

Supplementary Figure 4: Bacterial RNA induces BCL6/IL-21 expression, ST and HKST equally induce TBET and FOXP3 expression.

From: Recognition of microbial viability via TLR8 drives TFH cell differentiation and vaccine responses

Supplementary Figure 4

(a) Purified CD14+ porcine monocytes were stimlated with bacterial RNA complexed with pLa, Pam3CSK4 (200ng/ml) or LPS (2ug/ml). Supernatatnts were collected after 24h and analyzed by ELISA (n=3, 4). Error bars, maximum and minimum (*; p<0.05, **; p<0.01, ***; p<0.001). (b, c) Tbet/FoxP3 expression was detected by flow cytometry in CD3+CD4+ T cells or CD3+CD4+CD8+ T cells from pigs immunized subcutaneously with saline (ctrl), live attenuated S. enterica serovar Typhimurium vaccine (ST) or heat killed ST (HKST). (d, e) Quantification of (b) and (c) respectively (n=6,5,5). One-way ANOVA with post hoc correction for multiple comparison. Error bars are mean ± SEM. Error bars are mean ± SEM. (*; p<0.05).

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