Supplementary Figure 5: Vaccination with ST induces B cell follicles with active germinal centers.
From: Recognition of microbial viability via TLR8 drives TFH cell differentiation and vaccine responses
(a) Sections of paraffin embedded spleen tissues were stained for KI67. Scale bars: upper panels; 5mm; lower panels; 500µm (b) Sections of paraffin embedded lymph nodes were stained for PAX5, KI67 and BCL-2. Scale bar= 200µm. (c) Co-immunofluorescence staining of PAX5 (red) and KI67 (green) on spleen section of pigs vaccinated with ST, HKST, or saline injected control animals (ctrl). Nuclei were counterstained with DAPI (blue). Control sections stained only with DAPI to control for autofluorescence are shown in the bottom row. Note the high level of autofluorescence of the red pulp (erythrocytes), whereas no interfering autofluorescence signal was detected in the lymphoid follicles. Scale bar; 50µm.
