Supplementary Figure 7: Altered clefts and cavities in the TLR8-G variant.
From: Recognition of microbial viability via TLR8 drives TFH cell differentiation and vaccine responses
(a, b) CASTp analysis showing 148 functional pockets of TLR8 Isoform B TLR8-A variant (a) and 135 for Isoform B TLR8-G truncated variant (b). Pockets are coloured differently. (c, d) Rear view of TLR8 structures. Isoform B TLR8-A variant (c) and Isoform B TLR8-G truncated variant (d) showing alteration of residues in pockets associated with dimerization. Surface diagram showing major functional clefts and cavities in TLR8. (e,f) Isoform B TLR8-A variant (e) and Isoform B TLR8-G truncated variant (f). Colours represent different clefts arranged according to volume from largest to smallest. The TLR8-G variant is 42.9% rigid (i.e., more flexible) compared to 58% for TLR8-A. (g) Comparison of the volumes of clefts between the two gene variants.
