Supplementary Figure 5: Lipid accumulation in dendritic cells alters metabolic profile and differs in LXR expression.

(a) Bone marrow chimeric mice were generated as described in Fig. 5. The levels of NP-specific antibodies were analyzed (a). Data are one representative experiment of two independent experiments with n=4 per group. (b-f) CD11c⁺ dendritic cells were isolated from the indicated mice and were analyzed for ECAR profile (b), glycolysis, glycolytic capacity (c), OCR profile (d), basal respiration, ATP production, and maximal respiration (e). Energy map of dendritic cells isolated from the indicated mice (f). Data are representative of three independent experiments with two technical replicates (n=5 per group). (g) BODIPY staining of each cell types. Data are one representative experiment of three independent experiments with n=5 per group. (h-j) Splenocytes from indicated mice were enriched with CD11c (h) and flow-sorted (i). The expression of LXRβ was examined by Immunoblot analysis. Quantified values of the band intensities are presented at the bottom of each blot. Cropped blot images (h). Quantitative RT-PCR analysis of the indicated gene were determined (i). Original gel image from Supplementary Fig. 6h (j). Data are one representative experiment of three independent experiments with n=5 per group. *, p<0.05; **, p<0.01 between the two groups except in a. (a) *, p<0.05; **, p<0.01; ***, p<0.001 in comparison with ApoE^WT. ^#, p<0.05; ^##, p<0.01 in comparison with WT^WT mice (unpaired Student’s t-test, mean+SEM).