Supplementary Figure 5: AhR does not impact dexamethasone-induced thymic involution and Cyp1a1 expression is independent of Ido1 and the microbiome in myeloid cells from lupus prone mice. | Nature Immunology

Supplementary Figure 5: AhR does not impact dexamethasone-induced thymic involution and Cyp1a1 expression is independent of Ido1 and the microbiome in myeloid cells from lupus prone mice.

From: Apoptotic cell–induced AhR activity is required for immunological tolerance and suppression of systemic lupus erythematosus in mice and humans

Supplementary Figure 5

(a) Thymic cell numbers were quantified in B6 mice, B6 mice treated with AhR inhibitor (CH223191), and LysM-AhR cKO mice 24h after dexamethasone administration (0.2 mg per mouse, i/p). Bar graph represents total thymic cell count. (b, c) DC and macrophages were FACS-sorted from the spleen of female, 12 week old mice of the mouse strain indicated on the basis of F4/80, CD11c, CD8α. Expression of mRNA for Cyp1a1 and Ido1 were measured by sqPCR. For a, b, c n=4 biologically independent samples per group. Bars represent the mean +/- the standard deviation. (d) 12 week old female R2B mice were kept on antibiotic water for two weeks. DC and macrophages were sorted as in b and mRNA for Cyp1a1 and Ido1 were measured. Bars represent the value of pooled samples from four mice. For all sqPCR analysis samples were normalized against Bactin. ***=P<0.001 as determined by two-sided Student’s t-test. All experiments were repeated two times with similar results.

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