Supplementary Figure 5: Study of DC progenitors and in vitro differentiation. | Nature Immunology

Supplementary Figure 5: Study of DC progenitors and in vitro differentiation.

From: Disruption of an antimycobacterial circuit between dendritic and helper T cells in human SPPL2a deficiency

Supplementary Figure 5

a) Example of the gating strategy for the rest of the figure. b) Example of the gating strategy, starting from live single cells in (a), from a healthy control and a SPPL2a-deficient patient used for Fig. 4a. c) Graphic representation of total CD34+ progenitors in healthy controls and patients, obtained from (b) showing the mean and S.D. d) Gating strategy, starting from live single cells in (a), for a control and a SPPL2a-deficient patient for the graphs shown in Fig. 4b. e) Graphic representation of CD34+HLA-DR– DC progenitors obtained from (d) showing the mean and S.D. f) Gating strategy of early pre-DCs in healthy controls and SPPL2a-deficient patients, starting from the last gate in (a), the data for which are represented in Fig. 4c.

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