Supplementary Figure 6: Bach TFs functions in the differentiation of HSPCs.

a,b, Results of the LSK transplantations with or without Bach1 overexpression. Recipient mice were analyzed after 2 weeks. Representative flow cytometry plots of mature cells (a) and proportions of mature erythroid and myeloid cells (b) (n = 4 mice per condition, each dot represents an individual mouse, data were obtained from one experiment with individual measurement). c, Relative expression levels normalized by Actb expression of indicated genes in Gr1⁺ cells from wild-type or DKO derived HSPCs in the competitive transplantation analysis (n = 4 mice per genotype, each dot represents an individual mouse, samples were obtained individually and RT-qPCR was performed as in one experiment). d, Flow cytometry analysis in the competitive transplantation to determine wild-type HSPCs or DKO HSPCs derived HSC/MPP/LMPP percentages. Data were representative from one experiment using two mice with similar results. Bar plots show mean ± s.e.m (b,c). Statistical analysis was done with two-tailed Student’s t test or Welch’s t test (b,c). No adjustments were made for multiple comparisons. **P < .01, **P < .001.