Supplementary Figure 4: TLR3 trafficking to the cell periphery depends on microtubule polymerization.
From: Combating herpesvirus encephalitis by potentiating a TLR3–mTORC2 axis
MEF/TLR3/Unc93B1 cells were untreated (UT) or treated for 2 h with a Nocodazole at 10 µM. Cells were left unstimulated (U), stimulated (PIC) with poly(I:C) at 25 μg/ml for 16 h or infected (HSV-1) with HSV-1 at an MOI of 10 for 6 h. TLR3 (green), LAMP-1 (red) and nuclei (blue) were stained and analyzed by confocal microscopy. The statistical analyses of the peripheral TLR3 and LAMP-1 are also shown. Statistical analysis was performed by one-way ANOVA and Tukey’s test. ***P < 0.001, Scale bar, 20 μm. These experiments were repeated more than three times
