Supplementary Figure 3: GPCR132 inhibition leads to reduction of the acid-induced expression of ICER-encoding mRNAs.
From: Tumor immunoevasion via acidosis-dependent induction of regulatory tumor-associated macrophages
Relative expression of mRNAs encoding ICER in BMDMs stimulated under acidic pHe and in the presence of the GPCR132 antagonist l-α-lysophosphatidylcholine (LPC). 1 × 106 BMDMs (n = 4 each condition) were plated in 24-well plate for 8 h (Ctrl.). BMDMs were stimulated under acidic pHe (pH 6.1) by adding the inorganic acid HCl. Additionally, 160 µM or 40 µM of the GPCR132 antagonist LPC was added to the culture. Icer expression was analyzed by qPCR. Individual values are plotted within the bar graph.
