Supplementary Figure 8: CTLA-4 and MDL-12 administration in B16 bearing B6 mice.
From: Tumor immunoevasion via acidosis-dependent induction of regulatory tumor-associated macrophages
Tumor growth of CTLA-4 antibody– and adenylate cyclase inhibitor MDL-12-treated and untreated (n = 8 per group) B16 bearing C57BL/6 J mice. 2 × 105 B16 melanoma cells were inoculated s.c. into C57BL/6 J mice. On day 6 after tumor cell inoculation, either 0.5 mg CTLA-4 antibody was injected i.v. or additionally 20 µM MDL-12 was injected s.c. peritumoral. The MDL-12 administration was repeated every 3 d. As a control, PBS was injected i.v. and s.c. peritumoral. Tumor size was measured over a time period of 19 d using a caliper. Tumor volume was calculated using the formula width2 × length × 0.5. Individual tumor growth curves are shown.
