Supplementary Figure 2: β-catenin signaling is activated in the IFN-γ-producing human Treg subset.
From: Activated β-catenin in Foxp3+ regulatory T cells links inflammatory environments to autoimmunity
(a) GSEA enrichment plots of four different Wnt signaling pathway gene sets between IFN-γSP vs. DN. (b) Flow cytometric analysis of TCF-1 expression on ex vivo Treg subpopulations relative to DN (n = 7 subjects). *P < 0.05, **P < 0.01 (one-way ANOVA with Tukey’s multiple comparisons test). (c) AXIN2 and TCF7 mRNA expression in IFN-γ+ and IFN-γ– human Treg populations assessed by DNA microarray (n = 8 subjects). P values were calculated by two-sided Student’s t-test. (d) CTNNB1 gene expression on Treg cells transduced with a non-targeted shRNA or a CTNNB1 shRNA and cultured for 5 days (n = 10 subjects). P values were calculated by two-sided Student’s t-test. (e) Frequency of IFN-γ and IL-10 positive cell number. Treg cells were stimulated with anti-CD3 and anti-CD28 in the presence of SGK1 inhibitor GSK650394 (SGK1-i), IL-12 (TH1), or IL-12 and GSK650394 (TH1 + SGK1-i) (n = 6 subjects) ***P < 0.001 (one-way ANOVA with Tukey’s multiple comparisons test). Data were represented as mean + /- SD.
