Supplementary Figure 7: The PTGER2–β-catenin loop is activated by high-salt stimulation.
From: Activated β-catenin in Foxp3+ regulatory T cells links inflammatory environments to autoimmunity
(a) PTGER2 expression assessed by RNA-seq on ex vivo Treg subpopulations (n = 8 subjects). (b) Flow cytometric analysis of PTGER2 in human Jurkat T cells. Human Jurkat T cells were prepared as in Supplementary Fig. 6c. (n = 4). **P < 0.01, ***P < 0.001 (one-way ANOVA with Tukey’s multiple comparisons test). (c) Flow cytometric analysis of active β-catenin in human Jurkat T cells. Human Jurkat T cells were transduced with a scramble shRNA or a PTGER2 shRNA and cultured in normal media (Control) or media supplemented with additional 40 mM NaCl (NaCl) for 120 h. (n = 4) *P < 0.05, **P < 0.01 (one-way ANOVA with Tukey’s multiple comparisons test). (d) Representative flow cytometric analysis of IFN-γ and IL-10 production in human Treg cells cultured in the normal media (Control) or media supplemented with additional 40 mM NaCl (NaCl) with anti CD3 (2 μg/ml) and different concentration of anti CD28 (1, 2, 5 μg/ml) for 96 h. Relative frequency of IFN-γ and IL-10 producing Treg cells are shown at the bottom (n = 4 subjects). *P < 0.05, **P < 0.01 (two-way ANOVA with Sidak’s multiple comparisons test). Data were represented as mean + /- SD.
