Supplementary Figure 7: Generation of S100a11 KO mice.
From: Alarmin S100A11 initiates a chemokine response to the human pathogen Toxoplasma gondii
a, A schematic diagram of the CRISPR/Cas9 strategy used to generate S100a11-deficient mice and primer design used in the study. Exons 2 and 3 of the S100a11 gene were targeted by two sgRNAs depicted as sg#9 and sg#3. b, Representative genotyping of the targeted alleles with a set of primers S100A11 wtF and S100A11 wtR that result in PCR products of 2.7 kb for WT mice and 557 bp for the S100a11 KO allele, as a result of deletion of exons 2 and 3.
