Supplementary Figure 8: S100A11 regulates monocyte recruitment during mucosal response to T. gondii.

a,b, WT and S100a11 KO mice (n = 5) were infected orally with T. gondii, and the presence of monocytes and neutrophils in small intestinal lamina propria (n = 3) was analyzed (a) and quantified by flow cytometry on day 7 after infection (b). c, CCL2 and IFN-γ secretion in small intestine. d, Parasite burden in small intestine (n = 5) was measured by qRT–PCR. e, Histological analysis of the small intestines (n = 5) of infected WT and S100a11 KO mice with 20 cysts of ME49 T. gondii on day 7 after infection. Image fields are representative of pathology in multiple tissue sections, and chosen sections were selected by blinded observation. f, Histological changes in the small intestine were analyzed on day 7 after infection based on an additive scoring system. The data shown (mean ± s.d.) are representative of three independent experiments. Each symbol represents an individual experimental sample, and unpaired two-tailed Student’s t test was used for statistical analysis; ns, not significant.