Supplementary Figure 6: Flow cytometric gating.
a, Cells were dissociated from lungs of WT mice 14 d after bleomycin injury and stained with SiglecF, CD11c, and MHC II antibodies. SiglecF+CD11c+ cells were sorted into MHCIIhi and MHClo populations, with the threshold defined by MHC II staining in an uninjured mouse. Representative data are shown. b, Lung cells were dissociated from CX3CR1CreERT2 / Rosa26-loxp-STOP-loxp-TdTomato mice induced with tamoxifen 1 d prior to and during bleomycin injury and stained with SiglecF and MHC II antibodies. Representative data are shown.
