Supplementary Figure 7: Depletion of the macrophage subpopulation during bleomycin fibrosis. | Nature Immunology

Supplementary Figure 7: Depletion of the macrophage subpopulation during bleomycin fibrosis.

From: Reference-based analysis of lung single-cell sequencing reveals a transitional profibrotic macrophage

Supplementary Figure 7

a, Fluorescence microscopy demonstrating ablation of macrophages detected by immunofluorescence for SiglecF and macrophage marker MerTK26 in areas of scar by second-harmonic (SH) imaging 21 d after bleomycin injury in wild-type mice (left) and tamoxifen-induced Cx3cr1CreERT2/Rosa26-loxp-STOP-loxp-Diptheria Toxin A mice (right), with quantification in five fields of view per mouse (n = 3 biologically independent mice per group). The Wilcoxon rank-sum test two-sided P value is presented. Scale bar, 50 μm. The box plot center line is the median, box limits are the upper and lower quartiles, and whiskers denote the largest and smallest values no more than 1.5 times the interquartile range from the limits. b, H&E staining 21 d after injury of wild-type mice (left) and tamoxifen-induced Cx3cr1CreERT2 / Rosa26-loxp-STOP-loxP-Diptheria Toxin A mice (right). Scale bar, 0.5 mm. Images are representative of three independent replicates with similar results.

Back to article page