Supplementary Figure 2: Peritoneal neutrophils and macrophages from naïve mice express low levels of Mcpt8 mRNA.

Mcpt8 mRNA expression relative to GAPDH in Gr-1⁺ CD11b⁺ neutrophils (Neu) and F4/80⁺ CD11b⁺ macrophages (Macs) that were sorted from the peritoneum of naïve Tnf^fl/fl mice and Basoph8 x Tnf^fl/fl mice (n = 3 mice/group). Mcpt8 gene expression was assessed by qPCR using FcεRIα⁺ CD49b⁺ c-Kit^– basophils that were sorted from bone marrow of Tnf^fl/fl mice as positive controls. Mcpt8 mRNA expression levels in neutrophils and macrophages were normalized against Mcpt8 mRNA expression levels found in bone marrow basophils (Bas). Data are shown as means + SEM with squares representing values for individual experiments. The qPCR data are representative of n = 3 independent experiments performed, each of which gave similar results.