Supplementary Figure 4: Leukocyte populations in Tnffl/fl mice and Basoph8 × Tnffl/fl mice.
From: Basophil-derived tumor necrosis factor can enhance survival in a sepsis model in mice
(a-d) Percentages of leukocytes (a) Basophil percentages (FcεRIα+ CD49b+ c-Kit–) in the live-cell population isolated from spleens (Tnffl/fl, n = 7; Basoph8 x Tnffl/fl, n = 9), bone marrow (Tnffl/fl, n = 4; Basoph8 x Tnffl/fl, n = 9), or blood (Tnffl/fl, n = 16; Basoph8 x Tnffl/fl, n = 8), as analyzed by flow cytometry. (b) Neutrophil percentages (Gr-1+ CD11b+) in the live-cell population isolated from spleen (Tnffl/fl, n = 6; Basoph8 x Tnffl/fl, n = 6), bone marrow (Tnffl/fl, n = 6; Basoph8 x Tnffl/fl, n = 7), or peritoneal fluids (Tnffl/fl, n = 7; Basoph8 x Tnffl/fl, n = 10), as analyzed by flow cytometry. (c) Macrophage percentages (F4/80+ CD11b+) in the live-cell population isolated from spleens (Tnffl/fl, n = 7; Basoph8 x Tnffl/fl, n = 10) or peritoneal fluids (Tnffl/fl, n = 3; Basoph8 x Tnffl/fl, n = 10), as analyzed by flow cytometry. Data in a-d are depicted as mean + SEM with circles representing values for individual mice. (d) Mast cell percentages (FcεRIα+ c-Kit+) in the live-cell population isolated from peritoneal fluids (Tnffl/fl, n = 4; Basoph8 x Tnffl/fl, n = 6), as analyzed by flow cytometry. (e-g) Absolute numbers of leukocytes. (e) Blood monocyte (Tnffl/fl, n = 13; Basoph8 x Tnffl/fl, n = 9) and eosinophil (Tnffl/fl, n = 9; Basoph8 x Tnffl/fl, n = 10) numbers. (f) Blood lymphocyte (Tnffl/fl, n = 20; Basoph8 x Tnffl/fl, n = 10) and neutrophil (Tnffl/fl, n = 21; Basoph8 x Tnffl/fl, n = 9) numbers. (g) Blood lymphocyte (Tnffl/fl, n = 9; Basoph8 x Tnffl/fl, n = 9) and neutrophil (Tnffl/fl, n = 10; Basoph8 x Tnffl/fl, n = 8) numbers at 24 h after moderate CLP. Data in e-g are depicted as mean + SEM with circles representing values for individual mice. P values by Mann-Whitney test.
