Supplementary Figure 8: Neutrophils, macrophages and mast cells from Basoph8 × Tnf^fl/fl mice express negligible YFP and Mcpt8 mRNA after CLP.

(a) Representative flow cytometry plots and cell frequencies in the peritoneal neutrophil, macrophage, mast cell and basophil populations obtained from Tnf^fl/fl mice (n = 3) and Basoph8^± x Tnf^fl/fl mice (n = 3) at 24 h after moderate CLP. Cells were immunostained for surface Gr-1 and CD11b for peritoneal neutrophils; with F4/80 and CD11b for macrophages; and with FcεRIα, c-Kit and CD49b for mast cells (FcεRIα⁺ c-Kit⁺) and basophils (FcεRIα⁺ CD49b⁺). (b) The specificity of Mcpt8 gene expression as assessed by single cell PCR detection in peritoneal neutrophils (Neu), macrophages (Macs), mast cells (MCs), and BM basophils (BMBas) obtained from Tnf^fl/fl mice and Basoph8^± x Tnf^fl/fl mice at 24 h after moderate CLP. The numbers above the bar graphs indicate the number of cells analyzed. Results are expressed as % of cells expressing transcripts for the PCR products analyzed/cell type; results were pooled from n = 3 sorting experiments.