Supplementary Figure 9: TNF ablation in basophils does not affect ROS production and phagocytosis in neutrophils and macrophages ex vivo.
From: Basophil-derived tumor necrosis factor can enhance survival in a sepsis model in mice
Neutrophil ROS production (a) and macrophage phagocytosis (b). Bone marrow neutrophils were loaded for 30 min at 37 °C with 1 μM of CM-H2DCFDA, which is a general oxidative stress indicator, and then activated with 100 ng/ml of PMA. Peritoneal macrophages were incubated with opsonized FITC-labeled E. coli (K-12 strain) bioparticles for 10 min at 37 °C. Cells were stained for neutrophil and macrophage-specific surface markers and flow cytometry analysis was performed immediately thereafter. Data in a and b are shown as means + SEM with squares showing values for individual mice (n = 4 for Tnffl/fl mice in a and b, and n = 3 and n = 4 for Basoph8 x Tnffl/fl mice in a and b, respectively). Data were pooled from n = 2 independent experiments.
