Supplementary Figure 5: Sustaining mTOR-HIF1 activity fails to prevent UCP2-induced anti- tumor responses.
From: Uncoupling protein 2 reprograms the tumor microenvironment to support the anti-tumor immune cycle
a, b, Total reactive oxygen species, (a) and mitochondrial reactive oxygen species (b) in 3F and 3F-UCP2 B16-OVA melanoma cell lines treated with control vehicle or doxycycline were measured by CM-H2DCFDA and MitoSox Red (MitoSox), respectively. (n = 9 in each group). c, Immunoblot analysis of 3F-UCP2 B16-OVA cell and dual inducible B16-OVA cell, which simultaneously overexpress flag-tagged UCP2 and stabilized myc-tagged HIF-1α, treated with or without Dox. See also Supplementary Fig. 7a. d, e, Tumor growth (d) and tumor weight (e) of dual inducible B16-OVA melanoma from mice treated with or without Dox. (Ctrl, N = 6; Dox, n = 10). Data are mean±s.e.m. and analyzed by unpaired, two-tailed Student’s t-test. Data are cumulative results from at least three independent experiments (a-b, d-e), or representative images of three independent experiments (c). Each symbol represents one individual mouse.
