Supplementary Figure 6: UCP2 induction enhances CD8⁺ T cell infiltration and normalizes tumor vessels.

a, Relative number of CD8a⁺ cells in tumor cores and margins of 3F and 3F-UCP2 B16-OVA melanomas from control or doxycycline treated co-engrafted mice. (ANOVA p values: Core, p<0.0001; Margins, p = 0.0202). b, Normalized individual vessel surface area in tumor cores and margins of indicated melanomas. (ANOVA p value: Core, p = 0.0028). c, relative mural SMA⁺ cell coverage of CD31⁺ tumor endothelial cells in the indicated melanomas. (ANOVA p value = 0.0001). Data are mean±s.e.m. and analyzed by one-way ANOVA with Tukey's Multiple Comparison test (a, b, c). d, Characterization of the tumor vasculature in the indicated tumors. Proportion of single VCAM-1⁺ or mural cell-covered (SMA⁺), double positive (SMA⁺VCAM-1⁺) and immature (SMA^negVCAM-1^neg) vessels were quantified. Endothelial cells were identified by staining for pan-endothelial marker CD31, Data are mean±s.e.m. (a, 3F, n = 5; 3F = Dox, n = 6; 3F-UCP2, n = 6; 3F-UCP2+Dox, n = 7. b-d, 3F, n = 5; 3F = Dox, n = 6; 3F-UCP2, n = 6; 3F-UCP2+Dox, n = 6). e, Representative images of immunofluorescent staining for indicated proteins and DNA of YUMM1.7-OVA melanomas from wild type, Rag1-KO or CD8⁺ T cell-depleted mice. Scale bar, 50 µm. f-h, Quantification of the data shown in e. Relative number of tumor infiltrating CD8a⁺ cells (f); relative single tumor vessel area (g); and relative mural SMA⁺ cell coverage of VE-cadherin⁺ blood vessels (h). Data are mean±s.d. and analyzed by two-way ANOVA with Tukey's Multiple Comparison test (f, g, h). *p ≤0.05; **p ≤0.01; ***p ≤0.001; ****p ≤0.0001. Sample size: e-h, each group (n = 5). Data are cumulative results from two independent experiments (a-d, f-g) and representative images of two independent experiments (e). Each symbol represents one individual mouse.