Supplementary Figure 5: Inducible deletion of Elmo1 in ongoing arthritis.
(a) Paw swelling of male and female Elmo1fl/flUbc-CreERt2 (n = 9 for males, n = 7 for females, green symbols) and littermate control (n = 12 for males, n = 10 for females, black symbols) mice with K/BxN serum transfer induced arthritis and tamoxifen administration (arrows). b) Paw swelling (left panel) and clinical scores (right panel) of Elmo1fl/fl (control, n = 5) and Ubc-CreERt2/Elmo1fl/fl (n = 3) mice with K/BxN serum transfer induced arthritis without tamoxifen administration. c) Schematic of the K/BxN serum transfer arthritis induction and treatment with tamoxifen to induce deletion of Elmo1 during ongoing arthritis. d) Paw swelling of male Ubc-CreERt2/Elmo1fl/fl (n = 3, green symbols) and littermate control (n = 6, black symbols) mice with K/BxN serum transfer induced arthritis and tamoxifen administration (arrows). e) ELMO1 protein expression in human peripheral blood neutrophils (duplicates are shown) and buffy coat cells from two different donors (1 and 2). ERK protein expression is used as a loading control. The blot was cropped to show relevant bands.
