Supplementary Figure 3: Identification of CD4⁺ T cell clusters in fetal intestines.

(a) Representative biaxial plots showing the gating strategy for T_N, CD161⁻ T_M, CD161⁺CD117⁻ T_M, CD161⁺CD117⁺ T_M and T_reg cell clusters derived from one fetal intestine analyzed by flow cytometry for expression of CD3, CD4, CD8a, CD25, CD45RA, CD45RO, CD117, CD127, CD161 and TCRγδ (n = 10). (b) Expression (log-normalized) of KIT as determined by single-cell RNA-seq analysis, presented as violin plots. Colors indicate different cell clusters. (c) Bar plot depicting cell frequencies for clusters identified by mass cytometry and RNA-seq.