Supplementary Figure 5: NLRX1 does not affect the production of type I IFN or inflammatory cytokines after L.m infection.
From: Listeria hijacks host mitophagy through a novel mitophagy receptor to evade killing
a-c, ELISA analysis of TNF in spleen (a) (day 0, n=3 and day 3, n=6) and serum (b) (day 3, n=6), and IL-1β in spleen (c) (day 0, n=4 and day 3, n=6) from Nlrx1fl/fl or Nlrx1fl/flLys2-cre mice after intraperitoneally L.m infection for 3 days. d-g, Q-PCR analysis of Tnf in spleen (d) and liver (e), Il6 in spleen (f), and Ifnb in spleen (g) from Nlrx1fl/fl or Nlrx1fl/flLys2-cre mice after L.m infection for 3 days. (day0, n=4 and day3, n=6). h, Western blot analysis of p-IκBα and p-P65 in WT or Nlrx1–/– PMs infected with L.m (MOI 5) for the indicated time points. i-m, Q-PCR analysis of Tnf (i) (n=3), Il6 (j) (n=3) and Ifnb (l) (n=3) or ELISA analysis of IL-6 (k) (n=4) and IL-1β (m) (n=4) of WT or Nlrx1–/– PMs after L.m infection for the indicated time points. Data are representative of three independent experiments (a-m) and represent mean ± SEM (a-g,i-m). Two-sided Student’s t-test was used to measure significance.
