Supplementary Figure 1: Single administration of NP-CT does not increase gut permeability or disrupt the mucosal epithelium. | Nature Immunology

Supplementary Figure 1: Single administration of NP-CT does not increase gut permeability or disrupt the mucosal epithelium.

From: BCR affinity differentially regulates colonization of the subepithelial dome and infiltration into germinal centers within Peyer’s patches

Supplementary Figure 1

a, NP-CT (10 μg) dissolved in 3% NaHCO3 or 3% NaHCO3 without NP-CT were given by gavage to wild type mice, indicated as ‘NP-CT’ and ‘Control’, respectively. 24 hours post antigen administration, mice were fed with FITC dextran or left untreated (negative control, NC). FITC fluorescence was measured in blood serum, 2.5 hours after FITC dextran administration. Data were pooled from two independent experiments with three mice in each group (n=6), bar represents mean. P<0.05, one-way ANOVA. b, Diagram representing the experimental protocol in (c-e). c-e, H&E histological section of duodenum (c), jejunum (d), and ileum (e) following NP-CT administration as indicated in (b). Scale bar, 200 μm. Two independent experiments with two mice in each group (n=4) were performed and showed similar results.

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