Supplementary Figure 4: Inhibition of NAMPT affects macrophage metabolism but does not modulate the frequency of immune cells in peritoneal lavage fluid or adipose tissue. | Nature Immunology

Supplementary Figure 4: Inhibition of NAMPT affects macrophage metabolism but does not modulate the frequency of immune cells in peritoneal lavage fluid or adipose tissue.

From: Inflammatory macrophage dependence on NAD+ salvage is a consequence of reactive oxygen species–mediated DNA damage

Supplementary Figure 4

Frequency of eosinophils (a), neutrophils (b), and dendritic cells (c) in peritoneal lavage 12 hours after i.p. administration of PBS, FK866, LPS or LPS and FK866 (n = 5 mice, representative of two independent experiments). (d) Basal ECAR and basal OCR of peritoneal macrophages isolated from mice given i.p. PBS, FK866, LPS or LPS and FK866 (n = 5 technical replicates derived from 5 pooled biological samples, representative of three independent experiments). (e) Basal ECAR and basal OCR of macrophages isolated from epididymal adipose tissue 12 hours after i.p. administration of PBS, FK866, LPS or LPS and FK866 (n = 5 technical replicates derived from 5 pooled biological samples, representative of two independent experiments). Frequency of macrophages (f) eosinophils (g), neutrophils (h), and dendritic cells (o) in epididymal adipose tissue and basal ECAR and basal OCR of macrophages isolated from epididymal adipose tissue 12 hours after i.p. administration of PBS, FK866, LPS or LPS and FK866 (n = 5 mice, representative of two independent experiments). Error bars are mean ± SEM. Data were analyzed by one-way ANOVA with Tukey’s multiple comparison test. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 000.1.

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