Supplementary Figure 6: Expression profiles define clusters of biological functions.

(a) A bipartite network demonstrates the coassociations among all nasal (light orange) and blood (light purple) modules (squares) and cell percentages (circles). Edges represent significant positive Pearson correlations >0.5 (FDR<0.05) and darker edges indicate higher correlations. Nodes are clustered according to their interconnectedness. Because respiratory epithelium and squamous cells were highly correlated, they were combined for this analysis. Several co-clustered networks are outlined in colors. Nasal epithelial (brown outline), nasal and blood eosinophil (pink outline) cells and pathways cluster towards the bottom of the network. These clusters include the upregulated core modules (Green squares) including pathways of multiple epithelial functions, eosinophil activation, and Th2 inflammation. Nasal macrophage and blood monocyte cells and modules cluster with one another (dark purple outline). This cluster includes blood and nasal interferon responses, the heat shock protein response, airway macrophage pathways, and blood monocyte pathways. Nasal lymphocyte pathways are not clustered with blood lymphocytes pathways (blue). Two core modules fall within the nasal lymphocyte cluster (green squares). Nasal neutrophil and blood neutrophil pathways do not cluster with one another (light green). (b) The heatmap shows the pairwise Pearson correlation coefficients between all nasal and blood module expression levels and nasal and blood cell percentages. Red indicates positive correlation and blue indicates negative correlation. Clusters represent coassociated modules and cell types. Abbreviated alphanumeric module names are used for brevity.