Supplementary Figure 7: Module validation demonstrates that module-cell associations and module coherence are reproducible.

(a) In the full set of 374 nasal samples, all genes that had been associated to a specific cell type during module generation, had statistically significant positive Pearson correlations with the same cell type (13,672 genes). (b) In the full set of 387 blood samples, all but 5 genes that had been associated to a specific cell type during module generation, had statistically significant positive Pearson correlations with the same cell type (13,311/13,316 genes). (c) Correlation of nasal module expression levels from the full set of 374 samples with cell differentials was assessed to determine a module’s final cell assignment. 27/52 nasal modules were assigned to one or more cell types. (d) Correlation of blood module expression levels from the full set of 387 samples with cell differentials to finalize a module’s cell assignment. 16/42 blood modules were assigned to one cell type. (e) The average Pearson correlation of genes assigned to each nasal module during module construction (146 samples) versus the average Pearson correlation of genes assigned to the same module within the full set of 374 samples. (f) The average Pearson correlation of genes assigned to each blood module during module construction (176 samples) versus the average Pearson correlation of genes assigned to the same module within the full set of 387 samples.