Supplementary Figure 5: Validation of STAT1 and STAT3 ChIP-Seq data. | Nature Immunology

Supplementary Figure 5: Validation of STAT1 and STAT3 ChIP-Seq data.

From: Activation of naïve CD4+ T cells re-tunes STAT1 signaling to deliver unique cytokine responses in memory CD4+ T cells

Supplementary Figure 5

(a) Representative peak alignment for STAT1 (top) an STAT3 (bottom) for selected target genes including: Irf9 (Chr14:55,602,633-55,611,556), Stat3 (Chr11:100,913,375-100,948,267), Nfat5 (Chr8:107,292,133-107,303,956, Il27ra (Chr8: 84,028,287-84,044,575), Stat3 (Chr11:100,931,206-100,942,360) and Cmtm6 (Chr9:114,729,054-114,740,438). (b) Panther analysis (http://pantherdb.org) for the differential expressed genes with a STAT1 or STAT3 binding in the promoter region for CD4+ TEM cells. (c) Enrichment of STAT1 and STAT3 to consensus binding motifs for specific transcription factors. Sequences were identified using MEME and STAMP/Jaspar software. For each condition, the two mostly highly predicted motifs are presented. Statistical significance of the motif prediction is shown as an E-value (MEME tool) (d) ChIP-qPCR analysis of STAT1, STAT3 and SP1 binding to promoter sequences from Irf1 and Socs3. CD4+ TN and TEM cells were treated with 20ng/ml IL-6 for 1 hour. For STAT1 and STAT3, ChIP-qPCR was normalised against binding to a control DNA sequence (NBS: non-binding sequence). To control for constitutive SP1 binding, analysis was normalised to untreated sample (UT) as described in Methods.

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